balb 3t3 murine fibroblast cell line  (ATCC)

Journal: Molecular Therapy Oncology

Article Title: Collagen depletion by pirfenidone enhances antitumor effect of oncolytic adenovirus against peritoneal metastases of gastric cancer

doi: 10.1016/j.omton.2025.201045

Figure Lengend Snippet: GC cells increase collagen 1 expression in fibroblasts and collagen increases proliferation of GC cells (A) Expressions of Col1A1, Col1A2, and ACTA2 mRNA in mouse gastric cancer (GC) cells (T3-2D), human GC cells (MKN45, MKN7, and NUGC4), mouse fibroblast cells (MEF), and human fibroblast cells (YS-1 and FEF3) are shown. All cells were analyzed by quantitative RT-PCR analysis. Data are expressed as the mean ± SD ( n = 3). (B) Expression of Col1A1 and Col1A2 mRNA in MEF (upper) and YS-1 (lower) cells after incubation with serum-free medium (SFM) as a control and conditioned medium (CM) of each GC cell type for 4 days. Cells were analyzed using quantitative RT-PCR analysis. Data are expressed as the mean ± SD ( n = 3). (C) Representative images of immunocytochemical staining of collagen 1 and α-SMA in MEF (left) and YS-1 (right) after incubation with normal medium and CM of each of the GC cells for 4 days. SFM was used as a control. The area index for each staining was evaluated by ImageJ software. Data are expressed as the mean ± SD ( n = 3). Scale bars, 50 μm. (D) Cell proliferation of T3-2D and MKN45 cells with and without 10% collagen stimulation for 72 h. Data are expressed as the mean ± SD ( n = 5). (E) Representative cellular morphological images of T3-2D and MKN45 cells with and without 10% collagen 1 stimulation for 72 h. Scale bar, 100 μm. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: A murine embryonic fibroblast (MEF) cell line was purchased from the American Type Culture Collection (Manassas, VA) and maintained in DMEM supplemented with 15% FBS.

Techniques: Expressing, Quantitative RT-PCR, Incubation, Control, Staining, Software

Journal: Molecular Therapy Oncology

Article Title: Collagen depletion by pirfenidone enhances antitumor effect of oncolytic adenovirus against peritoneal metastases of gastric cancer

doi: 10.1016/j.omton.2025.201045

Figure Lengend Snippet: Collagens and fibroblasts inhibit oncolytic virus penetration and reduce antitumor effects (A) T3-2D, MKN45, MEF, and YS-1 cells were infected with OBP-702 at the indicated MOIs for 3 days. Cell viability was assessed using the XTT assays. Cell viability was calculated relative to that of the mock-infected cells, which were set as 1.0. Data are expressed as the mean ± SD ( n = 5). (B) T3-2D and MKN45 cells were infected with OBP-702 at the indicated MOIs for 3 days with or without 10% collagen containing medium. Cell viability was assessed using the XTT assays. Cell viability was calculated relative to that of the mock-infected cells, which were set as 1.0. Data are expressed as the mean ± SD ( n = 5). (C) Representative cellular morphological images of T3-2D and MKN45 cells infected with 100 MOI of OBP-702 with or without 10% collagen stimulation for 72 h. Scale bars, 100 μm. (D) Representative spheroid morphological images of T3-2D cells infected with 20 MOI of OBP-702 or MKN45 cells infected with 50 MOI of OBP-702 with or without 2% collagen stimulation for 7 days. Scale bars, 100 μm. (E) ATP cell viability assay of T3-2D and MKN45 spheroids with or without 2% collagen stimulation for 7 days after infection with OBP-702 (20 MOI or 50 MOI). Data are expressed as the mean ± SD ( n = 5). (F) Representative images of mono-spheroids and co-cultured spheroids infected with OBP-401 (100 MOI) for 48 h. T3-2D and MKN45 cells were labeled with red cell trackers and MEF and YS-1 cells with blue cell trackers. Scale bars, 50 μm. ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: A murine embryonic fibroblast (MEF) cell line was purchased from the American Type Culture Collection (Manassas, VA) and maintained in DMEM supplemented with 15% FBS.

Techniques: Virus, Infection, Viability Assay, Cell Culture, Labeling

Journal: ACS Nano

Article Title: Toward a ToxAtlas of Carbon-Based Nanomaterials: Single-Cell RNA Sequencing Reveals Initiating Cell Circuits in Pulmonary Inflammation

doi: 10.1021/acsnano.5c12054

Figure Lengend Snippet: Mesenchymal activation supports CBN-induced acute inflammation (a). UMAP embedding illustrates cell type identity for the mesenchyme niche. (b). Relative cell frequency shows treatment-dependent effects, and lipofibroblasts (Lipofibro) are the most abundant fibroblast cell types. (c). Boxplot of treatment-dependent activation of the hallmark signaling pathway “TNF-a signaling via NFKB” in lipofibroblasts. Connectomes based on induced differential gene expression (DGE) analysis (treatment vs sham) show computationally inferred cellular communication strength in response to DWCNT (d) and MWCNT (e). For each circle plot, edge weight and color represent the number of ligand–receptor pairs between interacting cell types. (f). Heatmap of cytokine gene expression related to pro-inflammatory response in mesenchyme. The expression of Ccl2 (g) and Ccl11 (h) by CBN exposure in CCL-206 cells after 6 measured by qPCR. CNP (50 μg/mL), DWCNT (50 μg/mL) and MWCNT (30 μg/mL) were used in the in vitro study. For in vitro experiments, data are shown as mean ± SEM ( n = 3), one-way ANOVA followed by Dunn’s multiple comparisons test was used for statistical analysis.

Article Snippet: Murine epithelial lung tissue cell lines (LA-4; cat. no. ATCC CCL-196; MLE-12, cat no. CRL-2110), murine fibroblast cell line (CCL-206; cat. no. Mlg 2908), and murine macrophage cell line (J774.1; cat. no. TIB-67) were purchased from American Type Culture Collection (ATCC) instructions.

Techniques: Activation Assay, Gene Expression, Expressing, In Vitro