murine fibroblast cell line l929  (ATCC)

Journal: ACS Omega

Article Title: Comparative Study of Chitosan-Pyrophosphate and Magnesium Hydroxide-Alginate Hybrid Nanoparticles: Physicochemical Properties and Cytocompatibility toward Vascular Calcification Applications

doi: 10.1021/acsomega.5c12883

Figure Lengend Snippet: (a) Cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-Mg­(OH) 2 -Alg at different concentrations. * p < 0.05 vs control; (b) cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-CS-PPi at different concentrations. * p < 0.05 vs control.

Article Snippet: The murine fibroblast cell line L929 (ATCC NCTC clone 929 [L cell, CCL-1]) was used for cytotoxicity assays.

Techniques: Control

Journal: Advanced Science

Article Title: Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

doi: 10.1002/advs.202519225

Figure Lengend Snippet: miRNA Binding efficiency and in vitro study on L929 cells. (A) Binding efficiency of miRNA‐loaded LNPs evaluated in different solvent conditions (methanol and water) and on different microfluidic channel material (PDMS and Glass); (B) Assessment of miRNA‐LNP integrity after sonification, heating, and combined sonification and heating (C) Cell proliferation analyzed by PrestoBlue assay after treatment with increasing doses of LNPs, free miRNA, and LNP‐miRNA complexes (red arrow indicates the volume selected for further studies) (n = 5 samples/group); (D) Percentage of live cells following treatment with miRNA, LNP, dialyzed LNP (LNP_dial), miRNA‐loaded LNP (LNP‐miRNA), and dialyzed miRNA‐loaded LNP (LNP‐miRNA_dial); (E) Quantification of total cell nuclei in each treatment group, including miRNA, LNP, LNP_dial, LNP‐miRNA, LNP‐miRNA_dial, and CTRL. For (E,F) Data represent mean ± SD of 9 analyzed fields from a single well (technical replicates).

Article Snippet: L929 murine fibroblast cell line (cat. no. 400260) was obtained from Cytion.

Techniques: Binding Assay, In Vitro, Solvent, Prestoblue Assay

Journal: Advanced Science

Article Title: Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

doi: 10.1002/advs.202519225

Figure Lengend Snippet: Time‐dependent intracellular localization of Cy3‐labeled miRNA relative to the cell nucleus in L929 fibroblasts. Representative confocal microscopy images showing Cy3‐labeled miRNA (yellow) and DAPI‐stained nuclei (blue) in L929 fibroblasts treated with free miRNA (A), LNP‐miRNA (B), or LNP‐miRNA_dial (C) at the indicated time points (30 min, 1 h, 2 h, 4 h, and 24 h). Cy3‐miRNA signal is predominantly cytoplasmic and localized in the perinuclear region, with formulation‐dependent differences in the timing and intensity of intracellular miRNA appearance. Scale bar: 50 µm.

Article Snippet: L929 murine fibroblast cell line (cat. no. 400260) was obtained from Cytion.

Techniques: Labeling, Confocal Microscopy, Staining, Formulation

Journal: Advanced Science

Article Title: Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

doi: 10.1002/advs.202519225

Figure Lengend Snippet: Endo‐lysosomal trafficking and intracellular redistribution of miRNA in L929 fibroblasts. (A) Schematic illustration of miRNA complexation with lipid nanoparticles (LNPs), cellular uptake via endocytosis, and subsequent trafficking through early and late endosomal and lysosomal compartments visualized using LysoTracker staining. (B) Representative confocal microscopy images of L929 fibroblasts treated with free miRNA, LNP‐miRNA, or dialyzed LNP‐miRNA_dial and imaged at 30 min, 1 h, 2 h, 4 h, and 24 h post‐treatment. Cy3‐labeled miRNA is shown in yellow, LysoTracker‐stained acidic compartments in red, and nuclei (DAPI) in blue. Scale bar: 50 µm.

Article Snippet: L929 murine fibroblast cell line (cat. no. 400260) was obtained from Cytion.

Techniques: Staining, Confocal Microscopy, Labeling

Journal: Advanced Science

Article Title: Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

doi: 10.1002/advs.202519225

Figure Lengend Snippet: Single‐cell correlation analysis of intracellular miRNA (Cy3, Alexa 546) and LysoTracker (Alexa 647) signal intensity in L929 fibroblasts treated with free miRNA (green), LNP‐miRNA (turquoise), or LNP‐miRNA_dial (blue) at different time points (30 min–24 h). Each dot represents an individual cell. Untreated L929 fibroblasts (red) are included as a control to define baseline LysoTracker signal and background miRNA fluorescence. The data represent measurements acquired from 16 fields of view per condition, collected from three independent samples per group.

Article Snippet: L929 murine fibroblast cell line (cat. no. 400260) was obtained from Cytion.

Techniques: Single Cell, Control, Fluorescence

Journal: RSC Advances

Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities

doi: 10.1039/d6ra01587e

Figure Lengend Snippet: Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells (NIH-3T3) following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).

Article Snippet: The murine colon carcinoma cell lines CT26, human colorectal cancer cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from ATCC (USA).

Techniques: Activity Assay, Inhibition, MTT Assay

Journal: Journal of Nanobiotechnology

Article Title: Injectable ROS-scavenging and NIR-responsive nanocomposite hydrogel for staphylococcus aureus-infected diabetic wound healing

doi: 10.1186/s12951-026-04306-4

Figure Lengend Snippet: Biological functionality and biocompatibility of the PTPM hydrogel. a ) Tube formation assay in HUVECs, demonstrating the strongest proangiogenic activity in the PTPM hydrogel group. b , c ) Quantitative analyses of branch number and total tube length further confirmed its superior vascularization potential( n = 3 per group). d ) Intracellular ROS levels in L929 fibroblasts cultured with different hydrogels revealed the most efficient ROS scavenging in the PTPM group, e , f ) supported by quantitative fluorescence intensity and area measurements. g ) Scratch-wound assays at 0 and 24 h indicated markedly enhanced cell migration with PTPM treatment( n = 3 per group), h ) corroborated by statistical analysis of migration rates( n = 3 per group). i ) Live/dead fluorescence staining of L929 cells on days 1, 3 and 5 showed excellent cytocompatibility across all groups, with the PTPM hydrogel promoting the greatest proliferation over time. j–l ) Quantitative assessments of fluorescence intensity, viability, and proliferation on days 1, 3 and 5 further verified enhanced cellular growth and biocompatibility in the PTPM group( n = 3 per group). m–p ) qPCR analysis of proinflammatory (IL-1, iNOS) and anti-inflammatory markers (IL-4, CD206) demonstrated that the PTPM hydrogel most effectively suppressed inflammatory responses and promoted an anti-inflammatory phenotype( n = 3 per group). All data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001.HUVECs, human umbilical vein endothelial cells; ROS, reactive oxygen species; qPCR, quantitative polymerase chain reaction; NS, normal saline; PVA, polyvinyl alcohol; TSPBA, N¹-(4-boronobenzyl)-N³-(4-boronophenyl)-N¹,N¹,N³,N³-tetramethylpropane-1,3-diamine; PGS, Palygorskite; MoS₂, molybdenum disulfide; PT, PVA-TSPBA hydrogel; PTM, PVA-TSPBA@MoS₂ hydrogel; PTPM, PVA-TSPBA@PGS/MoS₂ hydrogel

Article Snippet: The murine fibroblast cell line L929 (Cat. No. CL-0137) and fetal bovine serum (FBS, Cat. No. 164210) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Tube Formation Assay, Activity Assay, Cell Culture, Fluorescence, Migration, Staining, Real-time Polymerase Chain Reaction, Saline